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- Title
Super‐resolution fluorescence microscopy for investigating bacterial cell biology.
- Authors
Carsten, Alexander; Wolters, Manuel; Aepfelbacher, Martin
- Abstract
Super‐resolution fluorescence microscopy technologies developed over the past two decades have pushed the resolution limit for fluorescently labeled molecules into the nanometer range. These technologies have the potential to study bacterial structures, for example, macromolecular assemblies such as secretion systems, with single‐molecule resolution on a millisecond time scale. Here we review recent applications of super‐resolution fluorescence microscopy with a focus on bacterial secretion systems. We also describe MINFLUX fluorescence nanoscopy, a relatively new technique that promises to one day produce molecular movies of molecular machines in action.
- Subjects
BACTERIOLOGY; FLUORESCENCE microscopy; BACTERIAL cells; CYTOLOGY
- Publication
Molecular Microbiology, 2024, Vol 121, Issue 4, p646
- ISSN
0950-382X
- Publication type
Article
- DOI
10.1111/mmi.15203