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- Title
Beneficial In Vitro Effects of a Low Myo -Inositol Dose in the Regulation of Vascular Resistance and Protein Peroxidation under Inflammatory Conditions.
- Authors
Rolnik, Agata; Olas, Beata; Szablińska-Piernik, Joanna; Lahuta, Lesław Bernard; Rynkiewicz, Andrzej; Cygański, Piotr; Socha, Katarzyna; Gromadziński, Leszek; Thoene, Michael; Majewski, Michał
- Abstract
Oxidative stress induces functional changes in arteries. Therefore, the effect of myo-inositol, a possible anti-inflammatory/antioxidant agent was studied on human plasma and rat thoracic arteries. Aortic rings from male Wistar rats (3 months of age) were incubated with myo-inositol (1, 10 and 100 μM, 120 min) and analyzed using the gas chromatography (GC) method. In another experiment, aortic rings were protected first with myo-inositol (1 µM, 60 min) and then subjected to a thromboxane receptor agonist (U-46619, 0.1 nM, 60 min). Therefore, these four groups under the following conditions were studied: (i) the control in the vehicle; (ii) myo-inositol; (iii) the vehicle plus U-46619; (iv) myo-inositol plus U-46619. The hemostatic parameters of human plasma and an H2O2/Fe2+ challenge for lipid and protein peroxidation were also performed. Myo-inositol was not absorbed into the pre-incubated aortic rings as measured by the GC method (0.040 µg/mg, p ≥ 0.8688). The effect of myo-inositol was more significant in the impaired arteries due to U-46619 incubation, which resulted in an improved response to acetylcholine (% Emax: 58.47 vs. 86.69), sodium nitroprusside (logEC50: −7.478 vs. −8.076), CORM-2 (% Emax: 44.08 vs. 83.29), pinacidil (logEC50: −6.489 vs. −6.988) and noradrenaline (logEC50: −7.264 vs. −6.525). This was most likely a possible response to increased nitric oxide release (×2.6-fold, p < 0001), and decreased hydrogen peroxide production (×0.7-fold, p = 0.0012). KCl-induced membrane depolarization was not modified (p ≥ 0.4768). Both the plasma protein carbonylation (×0.7-fold, p = 0.0006), and the level of thiol groups (×3.2-fold, p = 0.0462) were also improved, which was not significant for TBARS (×0.8-fold, p = 0.0872). The hemostatic parameters were also not modified (p ≥ 0.8171). A protective effect of myo-inositol was demonstrated against prooxidant damage to human plasma and rat thoracic arteries, suggesting a strong role of this nutraceutical agent on vasculature which may be of benefit against harmful environmental effects.
- Subjects
THERAPEUTIC use of antioxidants; PROTEIN metabolism; IN vitro studies; BIOLOGICAL models; INFLAMMATION; ANTI-inflammatory agents; ANIMAL experimentation; CELL receptors; ORGANIC compounds; VASCULAR resistance; TREATMENT effectiveness; RATS; GAS chromatography; ACETYLCHOLINE; OXIDATIVE stress; THORACIC arteries; DESCRIPTIVE statistics; HEMOSTATICS; NITRIC oxide; INOSITOL; LIPID peroxidation (Biology); SODIUM nitroferricyanide; HYDROGEN peroxide
- Publication
Nutrients, 2022, Vol 14, Issue 5, pN.PAG
- ISSN
2072-6643
- Publication type
Article
- DOI
10.3390/nu14051118