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- Title
Topical Application of A New Herbal Complex, NI-01, Ameliorates House Dust Mite-Induced Atopic Dermatitis in NC/Nga Mice.
- Authors
Jin, Seong Eun; Ha, Hyekyung; Yoo, Sae-Rom; Jeon, Woo-Young; Lee, Nari; Lee, Mee-Young; Choi, Susanna; Jang, Ji-Hye; Park, Eunsook; Kim, Sukkyoung; Seo, Chang-Seob
- Abstract
Atopic dermatitis (AD) is a chronic inflammatory skin disease characterized by pruritus and cutaneous dry skin. Here, we investigated whether topical application of NI-01 composed of six herbal medicines has a therapeutic effect on AD in vivo. Twelve marker compounds of NI-01 were analyzed by high-performance liquid chromatography with a photodiode array detector for quality control. To induce AD, house dust mite extract was applied to the shaved dorsal skin and ear surfaces of NC/Nga mice twice a week for 6 weeks. NI-01 (1, 2, or 4 mg/mouse) was applied daily to the site for experiment periods. The coefficient of determination of each compound showed good linearity (≥ 0.9999). The recovery rate of the 12 marker components was 96.77%–105.17%; intra and interday precision and repeatability were ≤ 1.40%. Topical application of NI-01 reduced house dust mite induced AD symptoms. The increased expressions of interleukin-4 and intercellular adhesion molecule-1 caused by house dust mites were markedly suppressed in NI-01-treated mice. Corticosterone levels significantly decreased, whereas serotonin levels increased with NI-01 application. These results suggest that NI-01 alleviates AD symptoms by inhibiting infiltration of inflammatory cells, thereby decreasing AD-related stress. NI-01 could be beneficial for the treatment of AD-like skin diseases.
- Subjects
INTERLEUKINS; HERBAL medicine; HIGH performance liquid chromatography; IN vivo studies; HOUSE dust mites; ANIMAL experimentation; SEROTONIN; ATOPIC dermatitis; MITE infestations; CELL adhesion molecules; CUTANEOUS therapeutics; MICE
- Publication
Nutrients, 2020, Vol 12, Issue 5, p1240
- ISSN
2072-6643
- Publication type
Article
- DOI
10.3390/nu12051240