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- Title
Evaluating the effect of arachidonic acid and eicosapentaenoic acid on induction of adipogenesis in human adipose-derived stem cells.
- Authors
Mostoli, Rezvan; Goudarzi, Farjam; Mohammadalipour, Adel; Khodadadi, Iraj; Goodarzi, Mohammad Taghi
- Abstract
Objective(s): Adipose tissue is one of the most important endocrine organs that liberates many metabolic mediators such as hormones, cytokines, and chemokines. Different types of fatty acids have key roles in adipogenesis. The aim of this study was to evaluate the effects of two essential fatty acids, including Arachidonic acid (AA) and Eicosapentaenoic acid (EPA), on the process of adipogenicity in human Adipose-Derived Stem Cells (hADSCs). Materials and Methods: After immunophenotyping of hADSCs by flowcytometry, they were differentiated into adipocytes and simultaneously exposed to 30 µM and 60 µM of AA and 25 µM and 50 µM of EPA. Further, along with the MTS assay, the activity of glycalaldehyde-3-phosphate dehydrogenase (GAPDH) was also measured. In addition, expression of lipid markers including peroxisome proliferator-activated receptor Δ2 (PPARΔ2) and glucose transporter 4 (GLUT4) was evaluated, and the neutral lipid contents were determined using Oil red O staining. Results: MTS evaluation showed a significant decrease in proliferation in all treatment groups compared to the control group. Based on oil red O staining, fat droplets in the AA treatment groups were higher than in controls. The expression of PPARΔ2 and GLUT4 genes and proteins increased in almost all AA and EPA groups compared to control. In addition, GAPDH activity was higher in AA groups than in the control group. In general, while different concentrations of EPA did not increase the adipogenic process compared to the control group, stimulation of differentiation to adipocytes was largely determined by the AA. Conclusion: The result indicates a positive effect of omega-6 versus omega-3 in stimulating the pathways of adipogenesis.
- Subjects
ARACHIDONIC acid; HUMAN stem cells; EICOSAPENTAENOIC acid; ESSENTIAL fatty acids; OLEIC acid
- Publication
Iranian Journal of Basic Medical Sciences, 2020, Vol 23, Issue 8, p1028
- ISSN
2008-3866
- Publication type
Article
- DOI
10.22038/ijbms.2020.41557.9819