We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
May-Hegglin anomaly in a dog.
- Authors
Flatland, Bente; Fry, Michael M.; Baek, Seung J.; Bahn, Jae H.; LeBlanc, Casey J.; Dunlap, John R.; Carroll, Roger C.; Kosiba, Deborah J.; Millsaps, Doris J.; Schleis, Stephanie E.
- Abstract
An 8-year-old female spayed Pug dog was presented for evaluation of cutaneous lesions occurring secondary to immunosuppressive treatment of presumed immune-mediated thrombocytopenia. Abnormal hematologic findings included persistent thrombocytopenia, macrothrombocytes, and variably shaped, often fusiform, blue cytoplasmic inclusions in neutrophils. May-Hegglin anomaly (MHA) was suspected based on the morphologic appearance of platelets and neutrophils. Examination of cells by transmission electron microscopy revealed normal platelet ultrastructure; neutrophil inclusions had features similar to those reported for inclusions in human MHA. Neutrophil function was within normal limits based on flow cytometric analysis. Thrombelastography indicated a prolonged clotting time ( r), and PlateletMapping showed a lack of response to 2 μM ADP compared with a moderate response in the control dog. Immunocytochemical staining of blood smears using 2 commercially available antibodies against MYH9 protein (nonmuscle myosin heavy chain II) yielded negative results. However, genomic DNA sequencing analysis of the dog's MYH9 gene identified a single point mutation, resulting in substitution of lysine for glutamine at the 1841 amino acid position; this mutation is identical to one identified in people with MHA. To our knowledge, this is the first report of an MYH9 mutation in the dog. MHA-associated macrothrombocytopenia may be mistaken for immune-mediated thrombocytopenia.
- Subjects
CASE studies; IMMUNOSUPPRESSIVE agents; NEUTROPHILS; THROMBELASTOGRAPHY; NUCLEOTIDE sequence; THROMBOCYTOPENIA
- Publication
Veterinary Clinical Pathology, 2011, Vol 40, Issue 2, p207
- ISSN
0275-6382
- Publication type
Article
- DOI
10.1111/j.1939-165X.2011.00320.x