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- Title
Poly (L-lactic acid) porous scaffold-supported alginate hydrogel with improved mechanical properties and biocompatibility.
- Authors
Jiaqi Chu; Shaodong Zeng; Liyang Gao; Groth, Thomas; Zhiwen Li; Junchao Kong; Mingyan Zhao; Lihua Li; Chu, Jiaqi; Zeng, Shaodong; Gao, Liyang; Li, Zhiwen; Kong, Junchao; Zhao, Mingyan; Li, Lihua
- Abstract
<bold>Purpose: </bold>Polymer porous scaffolds and hydrogels have been separately employed and explored for a wide range of applications including cell encapsulation, drug delivery, and tissue engineering.<bold>Methods: </bold>In this study, a three-dimensional poly (L-lactic acid) (PLLA) scaffold with interconnected and homogeneously distributed pores was fabricated to support the alginate hydrogel (Alg). The gels were filled into the porous scaffold, which acted as an analogue of native extracellular matrix (ECM) for entrapment of cells within a support of predefined shape. The mechanical strength of the composite scaffold was characterized by compression testing. The chondrocyte behavior in the scaffold was determined by inverted microscopy, scanning electron microscopy (SEM) and MTT viability assay. The repair efficiency of such a composite scaffold was further investigated in dog spinal defects by histological evaluation after implantation for 4 weeks.<bold>Results: </bold>Results showed that the composite scaffold possessed superior mechanical properties and hierarchical porous structure in comparison to pure Alg. Cell culture revealed that the cells presented a specific cartilage status in the composite scaffold in line with higher adherence and proliferation ratio. The histological analyses suggested that the composite scaffold substantially promotes its integration in the host tissue accompanied with a low inflammatory reaction and new tissue formation.<bold>Conclusions: </bold>The method thus provides a useful pathway for scaffold preparation that can simultaneously achieve suitable mechanical properties and good biocompatibility.
- Subjects
ALGINATES; ANIMAL experimentation; BIOMEDICAL materials; CARTILAGE; CARTILAGE cells; CULTURE media (Biology); DOGS; EXTRACELLULAR space; PHARMACEUTICAL gels; LACTIC acid; MATERIALS testing; PHYSICS; POLYMERS; TISSUE engineering; ACYCLIC acids
- Publication
International Journal of Artificial Organs, 2016, Vol 39, Issue 8, p435
- ISSN
0391-3988
- Publication type
journal article
- DOI
10.5301/ijao.5000516