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- Title
The Clostridium difficile proline racemase is not essential for early logarithmic growth and infection.
- Authors
Wu, Xiaoqian; Hurdle, Julian G.
- Abstract
Proline racemase (PrdF), which is important for energy metabolism via the Stickland pathway and is unique to certain clostridia, was investigated as a potential anti- Clostridium difficile target by examining its effects on the growth and virulence of C. difficile. Inactivation of PrdF by insertional mutagenesis did not affect early logarithmic growth but only attenuated growth in the mid- and late logarithmic phases. There was no effect on virulence in vivo, suggesting that PrdF is also not required for C. difficile infection. These findings indicate that PrdF as well as other enzymes encoded by the proline reductase operon are all nonessential and are unsuitable targets for anti- C. difficile drug discovery.
- Subjects
CLOSTRIDIUM disease treatment; CLOSTRIDIOIDES difficile; RACEMASES; ENERGY metabolism; MICROBIAL virulence; MUTAGENESIS; ANTIBACTERIAL agents
- Publication
Canadian Journal of Microbiology, 2014, Vol 60, Issue 4, p251
- ISSN
0008-4166
- Publication type
Article
- DOI
10.1139/cjm-2013-0903