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- Title
Conditional cell ablation by tight control of caspase-3 dimerization in transgenic mice.
- Authors
Mallet, Vincent O.; Mitchell, Claudia; Guidotti, Jacques-Emmanuel; Jaffray, Patrick; Fabre, Monique; Spencer, David; Arnoult, Damien; Kahn, Axel; Gilgenkrantz, Hélène
- Abstract
Studying the effects of the loss of a specific cell type is a powerful approach in biology. Here we present a method based on the controlled activation of the apoptotic machinery. We expressed a modified caspase-3containing chemical inducer of dimerization (CID)-binding sites in the livers of transgenic mice. In the absence of CID, no liver injury was detectable, underlining the absence of leakage in our system. In contrast, injection of the CID produced activation of the chimeric caspase-3, which led to a dose-dependent pure hepatocyte ablation with subsequent regeneration. This method is effective in both growing and nongrowing cells, and is therefore applicable to a wide range of cells and tissues. Moreover, because apoptosis has been described in numerous pathological circumstances, this system is useful for generating mouse models of human disorders as well as for studying the recovery or regeneration of tissues after cell loss.
- Subjects
APOPTOSIS; BINDING sites; TRANSGENIC mice
- Publication
Nature Biotechnology, 2002, Vol 20, Issue 12, p1234
- ISSN
1087-0156
- Publication type
Article
- DOI
10.1038/nbt762