We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
MOLECULAR BASIS OF ATTENUATION IN RECENTLY CIRCULATING PPRV IN RAJASTHAN.
- Authors
Galav, Alka; Maherchandani, Sunil; Galav, Vikas; Dadhich, Abhilasha
- Abstract
The Genomic and Antigenomic Promoter (GP and AGP) regions of peste des petits ruminants virus (PPRV) are composed of 107 bp and 109 bp, respectively. They play crucial role in replication and transcription of PPRV genome and in its pathogenesis. We attempted amplification of GP and AGP of PPRV from different field samples of sheep and goat origin, using 3' and 5' RACE PCR. The GP region was successfully amplified though, we could not amplify AGP region of the field isolate probably due to fast degradation of terminal region of RNA genome. Subsequently, amplicons were sequenced and compared with corresponding sequences of standard PPR vaccine strain (PPRV/Sungri96 strain) to identify potential base substitutions. The Multiple Sequence Alignment (MSA) of the GP region of vaccine strain PPRV/Sungri/96 (KJ867542.1), PPRV isolates (Bikaner-Khajuwala: Sheep origin and Hanumangarh-Nohar: Goat origin) from field samples and published sequences of Indian/PPRV strains (KX033350.1, KT270355.1, KT860063.1 KT860064.1 and KT860065.1) were undertaken by Clustal W in MEGA7. On comparative evaluation with the PPRV/Sungri/96, 4 base substitutions were observed in it; these were at position 5 (A & gt; G), 24 (A & gt; C), 47 (A & gt; G) and 102 (C & gt; T) in GP region. We hypothesized that base mutations found in the genomic promoter regions may be associated with attenuating properties of PPRV vaccine strain.
- Subjects
RAJASTHAN (India); PESTE des petits ruminants; REVERSE genetics; SEQUENCE alignment
- Publication
Veterinary Practitioner, 2019, Vol 20, Issue 2, p240
- ISSN
0972-4036
- Publication type
Article