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- Title
Distinguishing isolates of Deladenus siricidicola, a biological control agent of Sirex noctilio, from North America and the Southern Hemisphere using PCR-RFLP.
- Authors
Leal, I.; Foord, B.; Davis, C.; de Groot, P.; Mlonyeni, X.O.; Slippers, B.
- Abstract
The woodwasp Sirex noctilio Fabricius, along with its obligate symbiotic fungus Amylostereum areolatum (Chaillet ex Fr.) Boidin, is amongst the most damaging invasive species to many commercial pine plantations. The most effective biocontrol agent for management of this woodwasp has been the nematode Deladenus siricidicola Bedding. Before this agent can be used in North America, answering key questions about its interaction with native siricids and other strains of the nematode is essential, as would be the need to track its spread after release. The aim of this study was to develop tools to differentiate between the North American D. siricidicola isolates and the Southern Hemisphere Kamona strain of this species. We sequenced a region from ribosomal DNA and the cytochrome oxidase subunit 1 and developed a PCR-RFLP method based on a single nucleotide polymorphism flanking a microsatellite sequence. These markers will be useful for science-based operational biocontrol of S. noctilio.
- Subjects
SOUTHERN Hemisphere; NORTH America; SIREX noctilio; BIOLOGICAL control of insects; RESTRICTION fragment length polymorphisms; POLYMERASE chain reaction; INSECT-plant relationships; ANIMAL species; PLANTATIONS
- Publication
Canadian Journal of Forest Research, 2012, Vol 42, Issue 6, p1173
- ISSN
0045-5067
- Publication type
Article
- DOI
10.1139/x2012-058