We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
The bacterial superantigen Staphylococcal enterotoxin B stimulates lymphocyte locomotor capacity during culture <em>in vitro</em>.
- Authors
Newman, I.; Wilkinson, P. C.
- Abstract
The bacterial superantigen Staphylococcal enterotoxin B (SEB) was investigated for its effects on lymphocyte locomotion in vitro. Culture of peripheral blood mononuclear cells (PBMC) for 24–72 hr in SEB (1-100 μg/ml) increased the proportion of lymphocytes in locomotor (polarized) morphology and capable of invading collagen gels, to the same extent as the established locomotor activator, anti-CD3 (α-CD3), though the conventional antigen, tetanus toxoid was ineffective. The cells responding to SEB were predominantly T cells. SEB had no effect on lymphocyte locomotion in short-term (45 min) assays, thus its effect is to stimulate growth-related locomotor capacity and it does not act as a chemoattractant. During culture of PBMC in SEB, the chemokines interleukin-8 (IL-8) and macrophage chemotactic protein-1 (MCP-1) were released into the culture medium. The presence of anti-IL-8, but not of anti-MCP-1, either during culture or added to SEB culture supernatants and tested in short-term assays, inhibited the development of polarization suggesting that IL-8, which is a lymphocyte chemoattractant, also plays a key role in SEB-induced locomotor activation. Among SEB-activated lymphocytes, CD45RO+CD45RA- lymphocytes showed enhanced locomotor responses, but a relation was not found between locomotor activity and the presence of cell surface CD69.
- Subjects
BACTERIAL antigens; LEUCOCYTE motility; ENTEROTOXINS; INTERLEUKINS; MACROPHAGES; IMMUNOLOGY
- Publication
Immunology, 1996, Vol 87, Issue 3, p428
- ISSN
0019-2805
- Publication type
Article
- DOI
10.1046/j.1365-2567.1996.491565.x