We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Evaluation of anti-tumorigenic activity of BP3B against colon cancer with patient-derived tumor xenograft model.
- Authors
Hye-Youn Kim; Jinhee Kim; Huyen Trang Ha Thi; Ok-Sun Bang; Won-Suk Lee; Suntaek Hong
- Abstract
Background: KIOM-CRC#BP3B (BP3B) is a novel herbal prescription that is composed of three plant extracts. Our preliminary study identified that BP3B exhibited potent anti-proliferative activity against various types of cancer cell lines in vitro. Because the in vivo anti-tumor effect of BP3B is not evaluated before clinical trial, we want to test it using patient's samples. Methods: To confirm the in vivo anti-cancer effect of BP3B, we used genetically characterized patient-derived colon tumor xenograft (PDTX) mouse model. Anti-cancer activity was evaluated with apoptosis, proliferation, angiogenesis and histological analysis. Results: Oral administration of BP3B significantly inhibited the tumor growth in two PDTX models. Furthermore, TUNEL assay showed that BP3B induced apoptosis of tumor tissues, which was associated with degradation of PARP and Caspase 8 and activation of Caspase 3. We also observed that BP3B inhibited cancer cell proliferation by down-regulation of Cyclin D1 and induction of p27 proteins. Inhibition of angiogenesis in BP3B-treated group was observed with immunofluorescence staining using CD31 and Tie-2 antibodies. Conclusion: These findings indicated that BP3B has a strong growth-inhibitory activity against colon cancer in in vivo model and will be a good therapeutic candidate for treatment of refractory colon cancer.
- Subjects
ANIMAL experimentation; CELL lines; COLON tumors; HERBAL medicine; IMMUNOHISTOCHEMISTRY; MEDICINAL plants; MICE; RECTUM tumors; RESEARCH funding; STATISTICAL sampling; STATISTICAL hypothesis testing; PLANT extracts; DATA analysis software; DESCRIPTIVE statistics; IN vitro studies; IN vivo studies
- Publication
BMC Complementary & Alternative Medicine, 2016, Vol 16, p1
- ISSN
1472-6882
- Publication type
Article
- DOI
10.1186/s12906-016-1447-8