We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Agrobacterium -mediated transformation of protocorm-like bodies in Cymbidium.
- Authors
Dong Chin; Kei-ichiro Mishiba; Masahiro Mii
- Abstract
Abstract  Genetically transformed plants of Cymbidium were regenerated after cocultivating protocorm-like bodies (PLB) with Agrobacterium tumefaciens strain EHA101 (pIG121Hm) that harbored genes for β-glucuronidase (gus), hygromycin phosphotransferase (hpt) and neomycin phosphotransferase II (nptII). PLB of three genotypes maintained in liquid new Dogashima medium (NDM), were subjected to transformation experiments. The PLB inoculated with Agrobacterium produced secondary PLB, 4 weeks after transfer onto 2.5 g Lâ1 gellan gum-solidified NDM containing 10 g Lâ1 sucrose, 20 mg Lâ1 hygromycin and 40 mg Lâ1 meropenem. Transformation efficiency was affected by genotype and the presence of acetosyringone during cocultivation. The highest transformation efficiency was obtained when PLB from the genotype L4 were infected and cocultivated with Agrobacterium on medium containing 100 μM acetosyringone. Transformation of the hygromycin-resistant plantlets regenerated from different sites of inoculated PLB was confirmed by histochemical GUS assay, PCR analysis and Southern blot hybridization.
- Subjects
AGROBACTERIUM; GENETIC polymorphisms; GENETIC research; ANTIBACTERIAL agents
- Publication
Plant Cell Reports, 2007, Vol 26, Issue 6, p735
- ISSN
0721-7714
- Publication type
Article
- DOI
10.1007/s00299-006-0284-5