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- Title
A newly established canine NK‐type cell line and its cytotoxic properties.
- Authors
Grudzien, Malgorzata; Pawlak, Aleksandra; Kutkowska, Justyna; Ziolo, Ewa; Wysokińska, Edyta; Hildebrand, Wojciech; Obmińska‐Mrukowicz, Bożena; Strzadala, Leon; Rapak, Andrzej
- Abstract
We established a canine natural killer (NK)‐type cell line called CNK‐89 derived from a dog with NK cell neoplasia. Immunophenotyping analysis showed positive staining for CD5, CD8, CD45, CD56, CD79a and NKp46, while negative for CD3, CD4, CD14, CD20, CD21, CD34, Thy1, IgG, IgM and MHCII. Polymerase chain reaction analysis revealed the presence of CD56, NKG2D, NKp30, NKp44, NKp46 and perforin, but the absence of CD16, Ly49 and granzyme B mRNA. Treating CNK‐89 cells with IL‐2 did not change the expression of activating receptors, TNFα and IFNγ secretion and cytotoxic activity, however, treatment with IL‐12 alone or in combinations with IL‐15, IL‐18 and IL‐21 caused an increase in granzyme B and CD16 mRNA, IFNγ secretion and cytotoxic properties of the CNK‐89 cell line.
- Subjects
CELL lines; KILLER cells; POLYMERASE chain reaction; IMMUNOPHENOTYPING
- Publication
Veterinary & Comparative Oncology, 2021, Vol 19, Issue 3, p567
- ISSN
1476-5810
- Publication type
Article
- DOI
10.1111/vco.12695