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- Title
Correlation of apparent diffusion coefficient values measured by diffusion MRI and MGMT promoter methylation semiquantitatively analyzed with MS-MLPA in patients with glioblastoma multiforme.
- Authors
Sunwoo, Leonard; Choi, Seung Hong; Park, Chul ‐ Kee; Kim, Jin Wook; Yi, Kyung Sik; Lee, Woong Jae; Yoon, Tae Jin; Song, Sang Woo; Kim, Ja Eun; Kim, Ji Young; Kim, Tae Min; Lee, Se ‐ Hoon; Kim, Ji ‐ Hoon; Sohn, Chul ‐ Ho; Park, Sung ‐ Hye; Kim, Il Han; Chang, Kee ‐ Hyun
- Abstract
Purpose: To retrospectively determine whether the apparent diffusion coefficient (ADC) values correlate with O6-methylguanine DNA methyltransferase (MGMT) promoter methylation semiquantitatively analyzed by methylation-specific multiplex ligation-dependent probe amplification (MS-MLPA) in patients with glioblastoma. Materials and Methods: The study was approved by the Institutional Review Board and was Health Insurance Portability and Accountability Act (HIPAA) compliant. Newly diagnosed patients with glioblastoma ( n = 26) were analyzed with an ADC histogram approach based on enhancing solid portion. The methylation status of MGMT promoter was assessed by methylation-specific polymerase chain reaction (MSP) and by MS-MLPA. MS-MLPA is a semiquantitative method that determines the methylation ratio. The Ki-67 labeling index was also analyzed. The mean and 5th percentile ADC values were correlated with MGMT promoter methylation status and Ki-67 labeling index using a linear regression model. Progression-free survival (PFS) was also correlated with the ADC values using Kaplan-Meier survival analysis. Results: The mean methylation ratio was 0.21 ± 0.20. By MSP, there were 5 methylated and 21 unmethylated tumors. The mean ADC revealed a positive relationship with MGMT promoter methylation ratio ( P = 0.015) and was also significantly different according to MSP-determined methylation status ( P = 0.011). Median PFS was significantly related with methylation ratio ( P = 0.017) and MSP-derived methylation status ( P = 0.025). A positive relationship was demonstrated between PFS and the mean ADC value ( P = 0.001). The 5th percentile ADC values showed a significant negative relationship with Ki-67 labeling index ( P = 0.036). Conclusion: We found that ADC values were significantly correlated with PFS as well as with MGMT promoter methylation status. We believe that ADC values may merit further investigation as a noninvasive biomarker for predicting treatment response. J. Magn. Reson. Imaging 2013;37:351-358. © 2012 Wiley Periodicals, Inc.
- Publication
Journal of Magnetic Resonance Imaging, 2013, Vol 37, Issue 2, p351
- ISSN
1053-1807
- Publication type
Article
- DOI
10.1002/jmri.23838