We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
AtMYB12 regulates flavonoids accumulation and abiotic stress tolerance in transgenic Arabidopsis thaliana.
- Authors
Wang, Feibing; Kong, Weili; Wong, Gary; Fu, Lifeng; Peng, Rihe; Li, Zhenjun; Yao, Quanhong
- Abstract
In plants, transcriptional regulation is the most important tool for modulating flavonoid biosynthesis. The AtMYB12 gene from Arabidopsis thaliana has been shown to regulate the expression of key enzyme genes involved in flavonoid biosynthesis, leading to the increased accumulation of flavonoids. In this study, the codon-optimized AtMYB12 gene was chemically synthesized. Subcellular localization analysis in onion epidermal cells indicated that AtMYB12 was localized to the nucleus. Its overexpression significantly increased accumulation of flavonoids and enhanced salt and drought tolerance in transgenic Arabidopsis plants. Real-time quantitative PCR (qRT-PCR) analysis showed that overexpression of AtMYB12 resulted in the up-regulation of genes involved in flavonoid biosynthesis, abscisic acid (ABA) biosynthesis, proline biosynthesis, stress responses and ROS scavenging under salt and drought stresses. Further analyses under salt and drought stresses showed significant increases of ABA, proline content, superoxide dismutase (SOD) and peroxidase (POD) activities, as well as significant reduction of HO and malonaldehyde (MDA) content. The results demonstrate the explicit role of AtMYB12 in conferring salt and drought tolerance by increasing the levels of flavonoids and ABA in transgenic Arabidopsis. The AtMYB12 gene has the potential to be used to enhance tolerance to abiotic stresses in plants.
- Subjects
ARABIDOPSIS thaliana; TRANSGENIC plants; GENETICS of plant stress; FLAVONOIDS; ABIOTIC stress
- Publication
Molecular Genetics & Genomics, 2016, Vol 291, Issue 4, p1545
- ISSN
1617-4615
- Publication type
Article
- DOI
10.1007/s00438-016-1203-2