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- Title
Dual inhibitors of urease and carbonic anhydrase-II from Iris species.
- Authors
Saleem, Muhammad; Hareem, Sumaira; Khan, Ajmal; Naheed, Suad; Raza, Muslim; Hussain, Riaz; Imran, Muhammad; Choudhary, M. Iqbal
- Abstract
Twenty seven (1–27) known natural organic compounds were isolated for first time from two species of Iris, i.e. loczyi and Iris unguicularis. The structures of these compounds were deduced from the spectral data of NMR, IR, and mass spectrogram. These were evaluated against urease and carbonic anhydrase inhibition studies. For carbonic anhydrase-II inhibition studies, these compounds were evaluated by biochemical mechanism based in vitro bio-assay. Some compounds showed significant inhibition against CA-II enzyme. Compartively, compound (12) showed IC50 value of 17.60 ± 0.08 μM against urease enzyme, while compound (3) was found to be most active against carbonic anhydrase-II, having an IC50 value of 66.27 ± 0.89 μM. Izalpinin (3), 5,7-dihydroxy-2′,6-dimethoxyisoflavone (9), 4′,5,7-trihydroxy-6-methoxyflavanone (16), 4′,5,7-trihydroxy-3′,8-dimethoxyflavanone (20), 8-methoxyeriodictyol (21), and mangiferin (26) were found to be dual inhibitors of both the enyzmes. The most active compounds were docked using Autodock Vina and i-GEMDOCK softwares. The docking and in-vitro results are in agreement which showed secondary interactions with the enzymes. The compounds can serve as therapeutic agents to treat urease and carbonic anhydrase associated disorders.
- Subjects
EURASIA; CARBONIC anhydrase; ORGANIC compounds; FLAVONOID glycosides; BENZENE derivatives; ENZYMES
- Publication
Pure & Applied Chemistry, 2019, Vol 91, Issue 10, p1695
- ISSN
0033-4545
- Publication type
Article
- DOI
10.1515/pac-2019-0407