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- Title
A low-pungency S3212 genotype of Capsicum frutescens caused by a mutation in the putative aminotransferase ( p- AMT) gene.
- Authors
Park, Young-Jun; Nishikawa, Tomotaro; Minami, Mineo; Nemoto, Kazuhiro; Iwasaki, Tomohiro; Matsushima, Kenichi
- Abstract
The purpose of this study was to identify the genetic mechanism underlying capsinoid biosynthesis in S3212, a low-pungency genotype of Capsicum frutescens. Screening of C. frutescens accessions for capsaicinoid and capsiate contents by high-performance liquid chromatography revealed that low-pungency S3212 contained high levels of capsiate but no capsaicin. Comparison of DNA coding sequences of pungent (T1 and Bird Eye) and low-pungency (S3212) genotypes uncovered a significant 12-bp deletion mutation in exon 7 of the p- AMT gene of S3212. In addition, p- AMT gene transcript levels in placental tissue were positively correlated with the degree of pungency. S3212, the low-pungency genotype, exhibited no significant p- AMT transcript levels, whereas T1, one of the pungent genotypes, displayed high transcript levels of this gene. We therefore conclude that the deletion mutation in the p- AMT gene is related to the loss of pungency in placental tissue and has given rise to the low-pungency S3212 C. frutescens genotype. C. frutescens S3212 represents a good natural source of capsinoids. Finally, our basic characterization of the uncovered p- AMT gene mutation should contribute to future studies of capsinoid biosynthesis in Capsicum.
- Subjects
PUNGENCY; TABASCO pepper; AMINOTRANSFERASES; CAPSAICINOIDS; HIGH performance liquid chromatography; GENETIC mutation
- Publication
Molecular Genetics & Genomics, 2015, Vol 290, Issue 6, p2217
- ISSN
1617-4615
- Publication type
Article
- DOI
10.1007/s00438-015-1071-1