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- Title
Pllans–II Induces Cell Death in Cervical Cancer Squamous Epithelial Cells via Unfolded Protein Accumulation and Endoplasmic Reticulum Stress.
- Authors
Montoya-Gómez, Alejandro; Rivera Franco, Nelson; Montealegre-Sanchez, Leonel Ives; Solano-Redondo, Luis Manuel; Castillo, Andrés; Mosquera-Escudero, Mildrey; Jiménez-Charris, Eliécer
- Abstract
Due to the lack of chemotherapeutic drugs that selectively affect cervical cancer cells, natural sources such as snake venom are currently being investigated for molecules with antitumor potential. Pllans–II, a phospholipase A2 type–Asp49 from Porthidium lansbergii lansbergii snake venom, induced cell death in a cervical cancer cell line—Ca Ski—related to dysfunction in the ability to resolve endoplasmic reticulum stress, evidenced by sub–expression of genes such as PERK, ERO1 PDIs, HSP70, and CHOP. Western blot analysis validated the last two genes′ sub–expression at the protein level. In addition, Pllans–II presented a dose–dependent cytotoxic effect on cancer cells and an insignificant effect on healthy endothelial cells (HUVEC). Additionally, Pllans–II inhibited cancer cells′ adhesion and migration capacity, induced cell cycle arrest in the G2/M phase, and induced apoptosis stimulated possibly by the extrinsic route. These results demonstrate for the first time that Pllans–II has an antitumor effect on a squamous epithelial cervical cancer cell line and represents a possible biotechnological tool for designing a prominent antitumor agent.
- Subjects
SQUAMOUS cell carcinoma; CERVICAL cancer; SNAKE venom; ENDOPLASMIC reticulum; CELL death; WESTERN immunoblotting; CELL cycle
- Publication
Molecules, 2022, Vol 27, Issue 19, p6491
- ISSN
1420-3049
- Publication type
Article
- DOI
10.3390/molecules27196491