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- Title
Unambiguous NMR Structural Determination of (+)-Catechin—Laccase Dimeric Reaction Products as Potential Markers of Grape and Wine Oxidation.
- Authors
Deshaies, Stacy; le Guernevé, Christine; Suc, Lucas; Mouls, Laetitia; Garcia, François; Saucier, Cédric
- Abstract
(+)-Catechin—laccase oxidation dimeric standards were hemi-synthesized using laccase from Trametes versicolor in a water-ethanol solution at pH 3.6. Eight fractions corresponding to eight potential oxidation dimeric products were detected. The fractions profiles were compared with profiles obtained with two other oxidoreductases: polyphenoloxidase extracted from grapes and laccase from Botrytis cinerea. The profiles were very similar, although some minor differences suggested possible dissimilarities in the reactivity of these enzymes. Five fractions were then isolated and analyzed by 1D and 2D NMR spectroscopy. The addition of traces of cadmium nitrate in the samples solubilized in acetone-d6 led to fully resolved NMR signals of phenolic protons, allowing the unambiguous structural determination of six reaction products, one of the fractions containing two enantiomers. These products can further be used as oxidation markers to investigate their presence and evolution in wine during winemaking and wine ageing.
- Subjects
LACCASE; GRAPES; WINE aging; TRAMETES versicolor; OXIDATION; PLEUROTUS ostreatus
- Publication
Molecules, 2021, Vol 26, Issue 20, p6165
- ISSN
1420-3049
- Publication type
Article
- DOI
10.3390/molecules26206165