We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Rescue of avian leukosis subgroup-J-associated acutely transforming viruses carrying different lengths of the v- fps oncogene and analysis of their tumorigenicity.
- Authors
Wang, Yixin; Fang, Lichun; Li, Jianliang; Li, Yang; Cui, Shuai; Sun, Xiaolong; Chang, Shuang; Zhao, Peng; Cui, Zhizhong
- Abstract
In our previous study, six subgroup J strains of avian leukosis virus (ALV-J)-associated acutely transforming viruses carrying different lengths of the v- fps oncogene, designated as Fu-J and Fu-J1-5, were isolated and characterized from fibrosarcomas in ALV-J-infected chickens. In the present study, the oncogenic potential of Fu-J and Fu-J1-5 was investigated using a reverse genetics technique. Six replication-defective viruses, named rFu-J and rFu-J1-5, were rescued with the replication-competent rescued ALV-J strain rSDAU1005 as a helper virus by co-transfection of chicken embryo fibroblast monolayers with infectious clone plasmids. Experimental bird studies were performed, demonstrating that only the rescued rFu-J virus carrying the complete v- fps oncogene with rSDAU1005 as the helper virus could induce acute fibrosarcoma after inoculation in specific-pathogen-free (SPF) chickens. These results provide direct evidence that the replication-defective acutely transforming Fu-J virus, with the complete v- fps oncogene, was associated with acute fibrosarcoma in chickens infected with ALV-J in the field, as reported previously.
- Subjects
AVIAN leukosis; CHICKEN diseases; ONCOGENIC viruses; FIBROSARCOMA; REVERSE genetics; VIRAL replication
- Publication
Archives of Virology, 2016, Vol 161, Issue 12, p3473
- ISSN
0304-8608
- Publication type
Article
- DOI
10.1007/s00705-016-3035-0