We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Prevention of Reactive Oxygen-Induced Endothelial Cell Injury by Blocking Its Process.
- Authors
Shimura, H.; Yamaguchi, M.; Kuzume, M.; Matsumiya, A.; Matsumoto, T.; Sakai, H.; Hatakeyama, T.; Nakano, H.; Kumada, K.; Midorikawa, T.; Yoshizawa, Y.; Sanada, Y.; Ohata, H.; Sakagam, H. i; Takeda, M.
- Abstract
Endothelial cell (EC) injury induced by reactive oxygen species (ROS) was investigated and effects of Ca[sup 2+] channel blockers, agents which elevate intracellular cAMP levels ([cAMP][sub i] ), and protein kinase inhibitors on H[sub 2] O[sub 2] -induced EC injury were analyzed using human umbilical vein EC cultures. Exposure to H[sub 2] O[sub 2] increased intracellular Ca[sup 2+] levels and decreased [cAMP][sub i] . Ca[sup 2+] channel blockers, [cAMP][sub i] -elevating agents, and protein kinase inhibitors significantly inhibited H[sub 2] O[sub 2] -induced EC injury. Data suggest that H[sub 2] O[sub 2] -induced EC injury is mediated by extracellular Ca[sup 2+] influx, intracellular cAMP efflux, and intracellular signaling, each of which is blocked by Ca[sup 2+] channel blockers, [cAMP][sub i] -elevating agents, or protein kinase inhibitors. It is suggested that ischemia/reperfusion injury induced by ROS may be prevented by Ca[sup 2+] channel blockers, [cAMP][sub i] -elevating agents, and protein kinase inhibitors.
- Subjects
REACTIVE oxygen species; CALCIUM antagonists; CYCLIC adenylic acid; PROTEIN kinases; HYDROGEN peroxide; ISCHEMIA; TREATMENT of reperfusion injuries; THERAPEUTICS
- Publication
European Surgical Research, 1999, Vol 31, Issue 5, p390
- ISSN
0014-312X
- Publication type
Article
- DOI
10.1159/000008717