We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Selectable marker recycling in the nonconventional yeast Xanthophyllomyces dendrorhous by transient expression of Cre on a genetically unstable vector.
- Authors
Zhang, Ning; Li, Jiaxin; Li, Fuli; Wang, Shi'an
- Abstract
Selectable marker recycling is a basic technique in bioengineering. However, this technique is usually unavailable in non-model microorganisms. In this study, we proposed a simple and efficient method for selectable marker recycling in the astaxanthin-synthesizing yeast Xanthophyllomyces dendrorhous. This method was based on a Cre-loxP system, in which the transient expression of the Cre recombinase was controlled by a genetically unstable vector independent of episomal plasmids and inducible promoters. The selectable markers in single-gene locus and multigene loci were removed along with the loss of the Cre vector with a ratio of 100% and 29%, respectively. The significance of the method was highlighted by the finding that stable autotrophic mutants were not readily obtained in X. dendrorhous. Comparative studies in X. dendrorhous and the non-homologous end joining dominant yeast Yarrowia lipolytica suggested that the method could be universally used in homologous recombination dominant yeasts.
- Subjects
XANTHOPHYLLOMYCES dendrorhous; YEAST; BIOENGINEERING; GENE expression; MICROBIAL genetics
- Publication
Applied Microbiology & Biotechnology, 2019, Vol 103, Issue 2, p963
- ISSN
0175-7598
- Publication type
Article
- DOI
10.1007/s00253-018-9496-1