We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Characterization of cell growth and starch production in the marine green microalga Tetraselmis subcordiformis under extracellular phosphorus-deprived and sequentially phosphorus-replete conditions.
- Authors
Yao, Chang-Hong; Ai, Jiang-Ning; Cao, Xu-Peng; Xue, Song
- Abstract
Microalgal starch is a potential feedstock for biofuel production. Nutrient stress is widely used to stimulate starch accumulation in microalgae. Cell growth and starch accumulation in the marine green microalga Tetraselmis subcordiformis were evaluated under extracellular phosphorus deprivation with initial cell densities (ICD) of 1.5, 3.0, 6.0, and 9.0 × 10 cells mL. The intracellular stored phosphorus supported cell growth when extracellular phosphorus was absent. The maximum starch content of 44.1 % was achieved in the lowest ICD culture, while the maximum biomass productivity of 0.71 g L day, starch concentration of 1.6 g L, and starch productivity of 0.30 g L day were all obtained in the culture with the ICD of 3.0 × 10 cells mL. Appropriate ICD could be used to regulate the intracellular phosphorus concentration and maintain adequate photosynthetic activity to achieve the highest starch productivity, along with biomass and starch concentration. The recovery of phosphorus-deprived T. subcordiformis in medium containing 0.5, 1.0, or 6.0 mM KHPO was also tested. Cell growth and starch accumulation ability could be recovered completely. A phosphorus pool in T. subcordiformis was shown to manipulate its metabolic activity under different environmental phosphorus availability. Though lower starch productivity and starch content were achieved under phosphorus deprivation compared with nitrogen- or sulfur-deprived conditions, the higher biomass and starch concentration make T. subcordiformis a good candidate for biomass and starch production under extracellular phosphorus deprivation.
- Subjects
MARINE algae; GROWTH of plant cells &; tissues; STARCH; EFFECT of phosphorus on algae; FEEDSTOCK; MICROALGAE; PLANT nutrients
- Publication
Applied Microbiology & Biotechnology, 2013, Vol 97, Issue 13, p6099
- ISSN
0175-7598
- Publication type
Article
- DOI
10.1007/s00253-013-4983-x