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- Title
Prevalence of ST1049-KL5 carbapenem-resistant Klebsiella pneumoniae with a bla<sub>KPC-2</sub> and bla<sub>NDM-1</sub> co-carrying hypertransmissible IncM1 plasmid.
- Authors
Liu, Hongmao; Xiang, Yating; Xiong, Mengyuan; Xiao, Xiao; Zhou, Junying; Tian, Hongpan; Chen, Qingsong; Li, Yirong
- Abstract
Infection caused by KPC and NDM carbapenemases co-producing Klebsiella pneumoniae (KPC_NDM_CRKP) poses serious public health concerns. Here, we elucidate the prevalence of a hypertransmissible lncM1 plasmid, pKPC_NDM, co-carrying blaKPC-2 and blaNDM-1 genes in sequence type 1049 K_locus 5 (ST1049-KL5) KPC_NDM_CRKP isolates. Genetic and clonal relatedness analyses using pulsed-field gel electrophoresis, single nucleotide polymorphism analysis and core genome multilocus sequence typing suggested clonal dissemination of ST1049-KL5 KPC_NDM_CRKP strains in our hospital. Whole genome sequencing identified an identical 76,517 bp- blaKPC-2 and blaNDM-1 genes co-carrying IncM1 plasmid pKPC_NDM and a pLVPK-like hypervirulent plasmid in all ST1049-KL5 KPC_NDM_CRKP isolates. pKPC_NDM shared 100% identity with a previously sequenced plasmid CRKP35_unnamed4, demonstrating high transferability in conjugation assay, with conjugation frequencies reaching 10-4 and 10-5 in Escherichia coli and K. pneumoniae recipients, respectively. It also maintained favorable stability and flexible compatibility, with retention rates exceeding 80% after 10 days of continuous passage, and could be compatible with pre-existing blaKPC- or blaNDM-carrying plasmids in recipient strains. This study summarizes the characteristics of KPC_NDM_CRKP outbreaks and highlights the importance of ongoing surveillance and infection control strategies to address the challenges posed by ST1049 K. pneumoniae strains. The study focuses on a blaKPC-2 and blaNDM-1 co-carrying hypertransmissible IncM1 plasmid in ST1049 CRKP strains, highlights the importance of ongoing surveillance and infection control strategies to address the challenges posed by ST1049 K. pneumoniae.
- Subjects
CARBAPENEM-resistant bacteria; KLEBSIELLA pneumoniae; KLEBSIELLA infections; PULSED-field gel electrophoresis; WHOLE genome sequencing; SINGLE nucleotide polymorphisms
- Publication
Communications Biology, 2024, Vol 7, Issue 1, p1
- ISSN
2399-3642
- Publication type
Article
- DOI
10.1038/s42003-024-06398-w