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- Title
Plasminogen activator inhibitor-1 is involved in interleukin-1β-induced matrix metalloproteinase expression in murine chondrocytes.
- Authors
Akihiro Moritake; Naoyuki Kawao; Kiyotaka Okada; Masayoshi Ishida; Kohei Tatsumi; Osamu Matsuo; Masao Akagi; Hiroshi Kaji
- Abstract
Objectives: Interleukin (IL)-1b and matrix metalloproteinases (MMPs) play important roles in the pathogenesis of osteoarthritis. On the other hand, plasminogen activator inhibitor-1 (PAI-1), an inhibitor of fibrinolysis, exerts functions in the pathogenesis of various diseases. However, the functional roles of PAI-1 in the chondrocytes have been still remained unknown. Methods: In the present study, we investigated the roles of PAI-1 in the effects of IL-1b on the chondrocytes using wild-type and PAI-1-deficient mice. Results: IL-1b significantly elevated PAI-1 mRNA levels in the chondrocytes from wild-type mice. PAI-1 deficiency significantly blunted the mRNA levels of TGF-b and IL-6 enhanced by IL-1b in murine chondrocytes. Moreover, PAI-1 deficiency significantly decreased the mRNA levels of MMP-13, -3 and -9 as well as MMP-13 activity enhanced by IL-1b in the chondrocytes. In addition, PAI-1 deficiency significantly reversed type II collagen mRNA levels suppressed by IL-1b in the chondrocytes. On the other hand, active PAI-1 treatment significantly enhanced the mRNA levels of MMP-13, -3 and -9 as well as decreased type II collagen mRNA levels in the chondrocytes from wild-type mice. Conclusion: We first demonstrated that PAI-1 is involved in MMP expression enhanced by IL-1b in murine chondrocytes. PAI-1 might be crucial for the cartilage matrix degradation and the impaired chondrogenesis by IL-1b in mice.
- Subjects
INTERLEUKIN-1; MATRIX metalloproteinases; OSTEOARTHRITIS; PLASMINOGEN activator inhibitors; CARTILAGE cells
- Publication
Modern Rheumatology, 2019, Vol 29, Issue 6, p959
- ISSN
1439-7595
- Publication type
Article
- DOI
10.1080/14397595.2018.1525018