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- Title
BAFFLE: A 3D Printable Device for Macroscopic Quantification of Fluorescent Bacteria in Space and Time.
- Authors
TARDÍO PI, CARLES; REYES-GONZÁLEZ, DANIELA; FERNÁNDEZ-DUQUE, ANDREA; FUENTES-HERNÁNDEZ, AYARI; SANTOS-ESCOBAR, FERNANDO; PEÑA-MILLER, RAFAEL
- Abstract
Despite the ubiquity and importance of microbial communities, understanding the population dynamics of mixed cultures in structured environments remains a fundamental problem in microbial ecology. Identifying bacterial strains within a complex microbial community represents a challenging technical problem. We describe a low-cost optomechanical device designed to acquire multi-channel timelapse images of bacterial colonies growing in agar plates. This device uses a system of addressable LEDs and fluorescence filters to estimate the spatio-temporal distribution of different fluorescently-tagged cells from time-lapse images obtained using a standard DSLR camera with a macro lens. We demonstrate the potential of this device with a range of applications from experimental microbiology.
- Subjects
THREE-dimensional printing; MICROBIAL communities; MICROBIAL ecology; OPTOMECHANICS; BACTERIAL colonies
- Publication
Journal of Open Hardware, 2022, Vol 6, Issue 1, p1
- ISSN
2514-1708
- Publication type
Article
- DOI
10.5334/joh.44