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- Title
纤维素酶高产菌筛选鉴定及酶学性质初步研究.
- Authors
李明华; 孟秀梅; 王成龙
- Abstract
A high cellulase-producing strain SP08 was screened from soil, and identified based on the morphology characteristics of colony and the ITS sequencing technology. Finally, the properties of cellulase produced by the strain were preliminarily determined by enzymatic reaction. The results showed that strain SP08 was screened out and identified as Trichoderma koningii, which had high cellulase production ability. The cellulase activity reached (18.54±0.75) U/ml when cultured for 60 h, and reached the highest (19.18±0.68) U/ml for 72 h. The results of enzymology studies showed that optimum temperature and pH of cellulase produced by strain SP08 were 50 ℃ and 5.5, respectively, with high stability at 20-50 ℃ and pH 4.0-6.0. Mg2+, Mn2+ and Ca2+ could increase cellulase activity, while Fe2+ and Cu2+ had inhibitory effects on cellulase.
- Subjects
CELLULASE; ENZYMOLOGY; TRICHODERMA; SOILS; CELLULOSE; COASTAL sediments
- Publication
China Brewing, 2021, Vol 40, Issue 8, p134
- ISSN
0254-5071
- Publication type
Article
- DOI
10.11882/j.issn.0254-5071.2021.08.024