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- Title
Hydroalcoholic Leaf Extract of Punica granatum , alone and in Combination with Calcium Hydroxide, Is Effective against Mono- and Polymicrobial Biofilms of Enterococcus faecalis and Candida albicans.
- Authors
Sousa, Monica Naufel; Macedo, Alessandra Teixeira; Ferreira, Gabriella Freitas; Furtado, Haryne Lizandrey Azevedo; Pinheiro, Aruanã Joaquim Matheus Costa Rodrigues; Lima-Neto, Lídio Gonçalves; Fontes, Valéria Costa; Ferreira, Rayana Larissa Pinheiro Soares; Monteiro, Cristina Andrade; Falcai, Angela; Gomes, Lillian Nunes; Bragança, Queila da Silva Rosa; Torres, Dennyse de Sousa Brandão; Galvão, Lívia Câmara de Carvalho; Holanda, Rodrigo Assuncao; Santos, Julliana Ribeiro Alves
- Abstract
Failures in endodontic treatments are mostly associated with the difficulty in eradicating microbes of the root canal system, highlighting the need to develop novel effective antimicrobials. Punica granatum (pomegranate) leaf hydroalcoholic extract may be a potential alternative in canal dressing, owing to its antimicrobial properties. The objective of this study was to evaluate the antimicrobial activity of hydroalcoholic leaf extract of Punica granatum (HEPg) alone or in combination with calcium hydroxide (Ca(OH)2) against Enterococcus faecalis and Candida albicans in isolation and in mono- and polymicrobial biofilms. Microdilution tests in broth and assays for inhibition of biofilm formation were carried out to evaluate the antimicrobial properties of HEPg and HEPg + Ca(OH)2 against Enterococcus faecalis and Candida albicans. The cytotoxicity of HEPg in HaCaT cells was evaluated by MTT assay. HEPg and HEPg + Ca(OH)2 exerted significant antimicrobial activity against planktonic cells and mono- and polymicrobial biofilms. The combination of Punica granatum extract with Ca(OH)2 appears to be a promising alternative in endodontic treatments, which could be tested in vivo to confirm the efficacy of this mixture in disinfecting root canal systems.
- Subjects
POMEGRANATE; ENTEROCOCCUS faecalis; CANDIDA albicans; CALCIUM hydroxide; BIOFILMS
- Publication
Antibiotics (2079-6382), 2022, Vol 11, Issue 5, p584
- ISSN
2079-6382
- Publication type
Article
- DOI
10.3390/antibiotics11050584