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- Title
Temperature-dependent ultrastructural changes in the cone interphotoreceptor matrix.
- Authors
Toshiyuki Fujiwara; Takeshi Yoshitomi
- Abstract
Abstract Purpose To determine the temperature-dependent ultrastructural changes of the cone matrix sheath, a possible critical factor in the adhesive strength binding photoreceptors and pigment epithelial cells. Methods Aqueous-insoluble interphotoreceptor matrix (IPM) was extracted by soaking fresh bovine retinas in acidic HEPES-buffered solution followed by treatment with 1% Triton X-100 to remove cone membrane fragments. The IPM preparations were then incubated in the same buffer solution at either 34°C, 37°C, or 40°C for 60 min. After labeling with peanut agglutinin, the samples were prepared for electron microscopy. Results The cone matrix sheath, specifically stained with peanut agglutinin, was markedly condensed at 34°C. It became more dispersed and composed of granular elements at 37°C. At 40°C, the matrix appeared as a network of fine filamentous structures. Conclusions These findings suggest changing temperatures can alter the molecular organization of the IPM and thus influence its retinal adhesion.
- Subjects
INTERPHOTORECEPTOR matrix; PHOTORECEPTORS; EPITHELIAL cells; RETINAL (Visual pigment); ELECTRON microscopy; BUFFER solutions; EYE diseases
- Publication
Japanese Journal of Ophthalmology, 2009, Vol 53, Issue 5, p536
- ISSN
0021-5155
- Publication type
Article