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- Title
Engineered, nucleocytoplasmic shuttling Cas13d enables highly efficient cytosolic RNA targeting.
- Authors
Gruber, Christoph; Krautner, Lea; Bergant, Valter; Grass, Vincent; Ma, Zhe; Rheinemann, Lara; Krus, Ariane; Reinhardt, Friederike; Mazneykova, Lyupka; Rocha-Hasler, Marianne; Truong, Dong-Jiunn Jeffery; Westmeyer, Gil Gregor; Pichlmair, Andreas; Ebert, Gregor; Giesert, Florian; Wurst, Wolfgang
- Abstract
The article discusses the development of an engineered Cas13d protein called Cas13d-NCS, which enables efficient targeting of cytosolic RNA. Cas13d is a CRISPR system used for manipulating RNAs, but its activity is limited to the nucleus. Cas13d-NCS overcomes this limitation by shuttling crRNAs from the nucleus to the cytosol, where they can bind and degrade target RNAs. The researchers demonstrated the effectiveness of Cas13d-NCS in degrading mRNAs and a self-replicating RNA from the Venezuelan equine encephalitis virus, as well as blocking the replication of various SARS-CoV-2 strains. The study suggests that Cas13d-NCS has the potential to be used as a programmable therapy against cytosolic RNA viruses.
- Subjects
NUCLEOCYTOPLASMIC interactions; RNA; VENEZUELAN equine encephalomyelitis; RNA polymerase II
- Publication
Cell Discovery, 2024, Vol 10, Issue 1, p1
- ISSN
2056-5968
- Publication type
Article
- DOI
10.1038/s41421-024-00672-1