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- Title
The kidney-tonifying and blood circulation-promoting recipes promote the osteogenic differentiation of bone mesenehymal stem cells by regulating microRNA-210.
- Authors
ZHANG Beiyuan
- Abstract
Objective To investigate the mechanism of osteogenic differentiation of bone mesenehymal stem cells (BMSCs) promoted by the kidney-tonifying and blood circulation-promoting recipes. Methods BMSCs of SD rats were isolated with adherent separation method and primarily cultured in vitro. Then BMSCs were divided into blank group, osteo4nduced group, and Chinese medicine group. At the 7th day and the 14th day, the mineralized nodules were stained using Alizarin red statining (ARS). The formation of mineralized nodules was observed using a converted microscope. The expression of miR-210 was detected using real-time PCR. Results Compared with that in blank group, the number of mineralized nodules in osteo4nduced group and Chinese medicine group increased significantly (P < 0. 01). After the intervention of osteogenic inducer and extraction of the kidney-tonifying and blood circulation-promoting recipes, the expression of miR-210 in both groups decreased significantly compared with that in blank group. And the expression in Chinese medicine group was much lower than that in osteo4nduced group. Conclusion The kidney-tonifying and blood circulation-promoting recipes can promote the osteogenic differentiation of BMSCs by down-regulating the expression of miRNA-210.
- Subjects
MESENCHYMAL stem cells; CELL differentiation; HERBAL medicine; MICRORNA; CHINESE medicine
- Publication
Chinese Journal of Osteoporosis, 2014, Vol 20, Issue 4, p396
- ISSN
1006-7108
- Publication type
Article
- DOI
10.3969/j.issn.1006-7108.2014.04.012