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- Title
Cloning, sequencing, and expression of the genomic DNA encoding the protein phosphatase cdc25 inDictyostelium discoideum.
- Authors
Mayanagi, T.; Maeda, Y.; Hirose, S.; Arakane, T.; Araki, T.; Amagai, A.
- Abstract
A genomic DNA (Dd-cdc25) encoding the protein phosphatase cdc25 was isolated from the cellular slime moldDictyostelium discoideum. TheDd-cdc25DNA sequence, with a length of 2,958 bp, encodes a protein consisting of 986 amino acid (aa) residues. The sequence shares significant identities with cdc25 from human, mouse,Xenopus,Drosophila, andShizosaccharomyces pombe, particularly at the C-terminal region including the catalytic site for phosphatase activity. The deducedDictyosteliumcdc25 protein (Dd-cdc25) has the highest molecular mass (109.9 kDa) in several cdc25 species so far reported and contains four regions consisting of unusually long asparagine repeats (22-31) in the sequence. Unexpectedly, however, Western blot analysis using a specific antibody raised against the C terminus (aa 892-986) of Dd-cdc25 demonstrated that the protein exists as a short form (56 kDa), which has the C-terminal active site of phosphatase, during the course ofDictyosteliumdevelopment. The Western blot analysis also revealed marked changes in the phosphorylated state of the Dd-cdc25, coupling with cellular development.
- Subjects
DICTYOSTELIUM discoideum; MOLECULAR cloning; DNA; GENES; PHOSPHATASES; NUCLEOTIDE sequence
- Publication
Development Genes & Evolution, 2004, Vol 214, Issue 10, p510
- ISSN
0949-944X
- Publication type
Article
- DOI
10.1007/s00427-004-0429-x