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- Title
KOMPONENTNA DIJAGNOSTIKA ALERGIJSKIH BOLESTI.
- Authors
KOBAL, IVA MRKIĆ; TURKALJ, MIRJANA
- Abstract
Component-resolved diagnosis is a new in vitro diagnostic method in allergology that determines level of specific IgE antibodies to a particular allergen component (allergen epitope). Up to now, for determination of specific IgE we used allergenic extracts. Allergenic extracts are mixtures of allergens and other proteins, polysaccharides and lipids made by extraction from natural sources. The need for better standardization of allergen extracts has led to the development of purified allergenic extracts or allergenic components. They can be obtained by isolation from natural sources (natural purified allergens) or by production of recombinant DNA technology (recombinant allergens). Detection of sIgE on allergenic components can be performed individually (one sample - one analysis, singleplex analysis), or multiple (multiplex analysis) on microarray. So far, in clinical practice, we found component- resolved diagnosis to be particularly useful in distinguishing between primary sensitization to nutritional allergen and cross reactivity among inhalational and nutritional allergens and in risk assessment and prediction of severity of allergic reaction. In addition to nutritional allergy, the use of component - resolved diagnosis is now recommended in the diagnosis of allergy to venom hypersensitivity, for multiple sensitizations on inhalational allergens prior to specific immunotherapy, as well as in diagnosis of idiopathic anaphylaxis. The aim of this paper is to present the principles of component - resolved diagnostics and the possibility of using it in allergic diseases diagnosis.
- Subjects
RECOMBINANT DNA; ALLERGENIC extracts; ALLERGIES; ALLERGENS; DIAGNOSIS
- Publication
Paediatria Croatica, Supplement, 2019, Vol 63, p194
- ISSN
1330-724X
- Publication type
Article