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- Title
Navigating the labyrinth: a guide to sequence-based, community ecology of arbuscular mycorrhizal fungi.
- Authors
Hart, Miranda M.; Aleklett, Kristin; Chagnon, Pierre‐Luc; Egan, Cameron; Ghignone, Stefano; Helgason, Thorunn; Lekberg, Ylva; Öpik, Maarja; Pickles, Brian J.; Waller, Lauren
- Abstract
• Data generated from next generation sequencing (NGS) will soon comprise the majority of information about arbuscular mycorrhizal fungal (AMF) communities. Although these approaches give deeper insight, analysing NGS data involves decisions that can significantly affect results and conclusions. This is particularly true for AMF community studies, because much remains to be known about their basic biology and genetics. • During a workshop in 2013, representatives from seven research groups using NGS for AMF community ecology gathered to discuss common challenges and directions for future research. Our goal was to improve the quality and accessibility of NGS data for the AMF research community. Discussions spanned sampling design, sample preservation, sequencing, bioinformatics and data archiving. • With concrete examples we demonstrated how different approaches can significantly alter analysis outcomes. Failure to consider the consequences of these decisions may compound bias introduced at each step along the workflow. • The products of these discussions have been summarized in this paper in order to serve as a guide for any researcher undertaking NGS sequencing of AMF communities.
- Subjects
INNER ear; MYCORRHIZAL fungi; SOIL fungi; MYCOLOGY; SHIMEJI mushrooms
- Publication
New Phytologist, 2015, Vol 207, Issue 1, p235
- ISSN
0028-646X
- Publication type
Article
- DOI
10.1111/nph.13340