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- Title
The approach to the mechanism of calcitonin gene-related peptide-inducing inhibition of food intake.
- Authors
Sun, J.-Y.; Jing, M.-Y.; Wang, J.-F.; Weng, X.-Y.
- Abstract
The aim of this study was to investigate the anorectic mechanism of calcitonin gene-related peptide (CGRP) in rats. Intraperitoneal injection of CGRP (50 μg/kg) resulted in decline (p < 0.05) in the food intake of rats at 0.5, 1, 2 and 4 h in comparison with saline control. Compared with saline-treated group, the levels of hypothalamic 3′,5′-cyclic adenosine monophosphate (cAMP) and plasma glucagon were increased (p < 0.05) in CGRP-treated group, but insulin level was decreased (p < 0.05). No significant changes (p > 0.05) in the plasma leptin were observed between two treatment groups. Calcitonin gene-related peptide injection down regulated (p < 0.05) both neuropeptide Y (NPY) and melanin-concentrating hormone (MCH) genes at mRNA levels, but up regulated (p < 0.05) the expression of cholecystokinin (CCK) gene. The correlations analysis showed that food intake was negatively correlated (p < 0.05) with CCK mRNA, cAMP and glucagon levels. Moreover, there existed negative correlations (p < 0.05) between MCH mRNA and glucagon levels, and positive correlations (p < 0.05) between insulin and leptin levels. The results showed that cAMP acting as the second messenger may play a vital role in the anorectic effects of CGRP. Calcitonin gene-related peptide could stimulate anorexigenic neuropeptides (i.e. CCK) and/or inhibit orexigenic neuropeptides (i.e. NPY and MCH) expression, and ultimately suppressed food intake that was functionally coupled to cAMP/PKA pathway activation.
- Subjects
CALCITONIN gene-related peptide; INGESTION; APPETITE loss; ADENOSINE monophosphate; INTRAPERITONEAL injections; MESSENGER RNA; LABORATORY rats
- Publication
Journal of Animal Physiology & Animal Nutrition, 2010, Vol 94, Issue 5, p552
- ISSN
0931-2439
- Publication type
Article
- DOI
10.1111/j.1439-0396.2009.00937.x