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- Title
Hypertonic saline inhibits airway smooth muscle contraction by inhibiting Ca<sup>2+</sup> sensitization.
- Authors
Liu, Xiao‐Cao; Wang, Qian; She, Yu‐Shan; Chen, Shu; Luo, Xi; Xu, Hao; Zang, Dun‐An; Zhang, Wen‐Jing; Qiu, Jun‐Ying; Liu, Bei‐Bei; Shen, Jinhua; Peng, Yong‐Bo; Zhao, Ping; Xue, Lu; Chen, Weiwei; Ma, Li‐Qun; Fu, Xiangning; Chen, Jingyu; Liu, Qing‐Hua; Yu, Meng‐Fei
- Abstract
The effects of hypertonic solution on airway smooth muscle ( ASM) contraction and the underlying mechanisms are largely unknown. We found that hypertonic saline ( HS) inhibited acetylcholine ( ACh)-induced contraction of ASM from the mouse trachea and human bronchi. In single mouse ASM cells ( ASMCs), ACh induced an increase in intracellular Ca2+ that was further enhanced by 5% NaCl, indicating that the HS-induced inhibition of ASM contraction was not mediated by a decrease in cytosolic Ca2+. The Rho-associated kinase ( ROCK) inhibitor Y-27632 relaxed ACh-induced precontraction of mouse tracheal rings. However, such inhibition was not observed after the relaxation induced by 5% NaCl. Moreover, the incubation of mouse tracheal rings with 5% NaCl decreased ACh-induced phosphorylation of myosin light chain 20 and myosin phosphatase target subunit 1. These data indicate that HS inhibits the contraction of ASM by inhibiting Ca2+ sensitization, not by decreasing intracellular Ca2+.
- Subjects
SMOOTH muscle contraction; HYPERTONIC saline solutions; CALCIUM; SENSITIZATION (Neuropsychology); ACETYLCHOLINE receptor inhibitors; THERAPEUTICS
- Publication
Clinical & Experimental Pharmacology & Physiology, 2017, Vol 44, Issue 10, p1053
- ISSN
0305-1870
- Publication type
Article
- DOI
10.1111/1440-1681.12807