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- Title
Sevoflurane postconditioning affects post-ischaemic myocardial mitochondrial ATP-sensitive potassium channel function and apoptosis in ageing rats.
- Authors
Jiang, Jing‐Jing; Li, Chao; Li, Heng; Zhang, Lei; Lin, Zong‐Hang; Fu, Bao‐Jun; Zeng, Yin‐Ming
- Abstract
This study investigated the effect of sevoflurane postconditioning on post-ischaemic cardiac function, infarct size, myocardial mitochondrial ATP-sensitive potassium channel (mito KATP) function and apoptosis in ageing rats to determine the possible mechanism underlying the cardioprotective property of sevoflurane. Ageing rat hearts were isolated and attached to a Langendorff apparatus. The hearts were then exposed or not to sevoflurane postconditioning in the presence or absence of 100 μmol/L 5-hydroxydecanoate (5- HD), a selective mito KATP inhibitor. The infarct size was measured by triphenyltetrazolium chloride ( TTC) staining. Mitochondrial morphology was observed by electron microscopy and scored using FlaMeng semiquantitative analysis. In addition, the expression levels of Bax, Bcl-2, and cytochrome-C (Cyt-C) were determined by Western blot analysis at the end of reperfusion. Sevoflurane postconditioning increased coronary flow, improved functional recovery, reduced Bax/Bcl-2 and Cyt-C phosphorylation levels, and decreased mitochondrial lesion severity and the extent of apoptosis. The protective effects of sevoflurane postconditioning were prevented by the mito KATP inhibitor 5- HD. Sevoflurane postconditioning significantly protected the function of ageing hearts that were subjected to ischaemia and reperfusion, and these protective effects were mediated by mito KATP opening.
- Subjects
SEVOFLURANE; PHYSIOLOGICAL effects of potassium channels; APOPTOSIS; RAT physiology; REPERFUSION; ANIMAL models in research; PHYSIOLOGY
- Publication
Clinical & Experimental Pharmacology & Physiology, 2016, Vol 43, Issue 5, p552
- ISSN
0305-1870
- Publication type
Article
- DOI
10.1111/1440-1681.12565