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- Title
Beneficial cilostazol therapeutic effects in mdx dystrophic skeletal muscle.
- Authors
Hermes, Túlio de Almeida; Macedo, Aline Barbosa; Fogaça, Aline Reis; Moraes, Luis Henrique Rapucci; Faria, Felipe Meira; Kido, Larissa Akemi; Cagnon, Valéria Helena Alves; Minatel, Elaine
- Abstract
This study evaluated the possible protective effects of cilostazol against myonecrosis in dystrophic diaphragm muscle in vivo, focusing on oxidative stress, the inflammatory response and angiogenesis. Young mdx mice, the experimental animal for Duchenne muscular dystrophy, received cilostazol for 14 days. A second group of mdx mice and a control group of C57 BL/10 mice received a saline solution. In the mdx mice, cilostazol treatment was associated with reduced loss of muscle strength (−34.4%), decreased myonecrosis, reduced creatine kinase levels (−63.3%) and muscle fibres stained for immunoglobulin G in dystrophic diaphragm muscle (−81.1%), and a reduced inflammatory response, with a decreased inflammatory area (−22%), macrophage infiltration (−44.9%) and nuclear factor- κB (−24%) and tumour necrosis factor- α (−48%) content in dystrophic diaphragm muscle. Furthermore, cilostazol decreased oxidative stress and attenuated reactive oxygen species production (−74%) and lipid peroxidation (−17%) in dystrophic diaphragm muscle, and promoted the up-regulation of angiogenesis, increasing the number of microvessels (15%). In conclusion, the present results show that cilostazol has beneficial effects in dystrophic muscle. More research into the potential of cilostazol as a novel therapeutic agent for the treatment of dystrophinopathies is required.
- Subjects
TREATMENT of Duchenne muscular dystrophy; SKELETAL muscle physiology; QUINOLONE antibacterial agents; CREATINE kinase; OXIDATIVE stress; LIPID peroxidation (Biology); THERAPEUTICS
- Publication
Clinical & Experimental Pharmacology & Physiology, 2016, Vol 43, Issue 2, p259
- ISSN
0305-1870
- Publication type
Article
- DOI
10.1111/1440-1681.12521