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- Title
Isolation and molecular characterization of feline panleukopenia viruses from Korean cats.
- Authors
Dong-Kun Yang; Yu-Ri Park; Yeseul Park; Sungjun An; Sung-Suk Choi; Jungwon Park; Bang-Hun Hyun
- Abstract
Feline panleukopenia virus (FPV) causes fatal leukopenia and severe hemorrhagic diarrhea in cats. Although FPV isolates have been reported worldwide from several animals, the biological and genetic features of South Korean FPVs remain unclear. We characterized molecularly South Korean FPV isolates. Crandell-Rees feline kidney (CRFK) cells were used to isolate FPV from 60 organ homogenates. The isolates were confirmed to be FPVs via analyses of cytopathic effects, immunofluorescence studies, electron microscopy, and polymerase chain reaction. Viral genetic analyses used the full VP2 sequences. Eight isolates propagated in CRFK cells were confirmed to be FPVs. All isolates yielded viral titers ranging from 104.5 to 106.0 TCID50 /mL 5 days after inoculation into CRFK cells and exhibited hemagglutination titers ranging from 27 to 212 (using pig erythrocytes). The Korean FPV isolates grew well in cat cells such as CRFK and Fcwf-4 cells. The FPV isolates were most similar to the KS42 strain isolated from a Korean cat in 2008. The FPV isolates will serve as useful antigens in future sero-epidemiological studies and will aid in the development of diagnostic tools.
- Subjects
CATS; FELINE panleukopenia virus; POLYMERASE chain reaction
- Publication
Korean Journal of Veterinary Research, 2022, Vol 62, Issue 1, p1
- ISSN
2466-1384
- Publication type
Article
- DOI
10.14405/kjvr.20210050