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- Title
Presence of an adipokinetic peptide in the corpus cardiacum of Dermaptera but not in the neurohaemal aorta, and chemical and functional identification of the peptide.
- Authors
Gäde, GerD.
- Abstract
AbstractCorpora cardiaca of the earwigs Labidura riparia and Forficula auricularia contain a substance that causes hyperlipaemia in migratory locusts and hypertrehalosaemia in the American cockroaches. A conspecific bioassay in L. riparia revealed that this factor is lipid-mobilizing. Isolation of the neuropeptide was achieved by single-step RP-HPLC. The primary structure of the earwig adipokinetic peptide was elucidated by automated Edman degradation in combination with matrix-assisted laser desorption/ionization mass spectrometry. It is a blocked octapeptide, pGlu-Val-Asn-Phe-Ser-Thr-Gly-Trp-NH2, previously denoted as Grb-AKH and first identified in Gryllus bimaculatus (Gäde & Rinehart 1987). The synthetic peptide co-chromatographed under various conditions with the native peptide and, in biological assays, resulted in lipid-mobilization in L. riparia when injected in low concentration.
- Subjects
EARWIGS; LABIDURIDAE; FORFICULA; BIOLOGICAL assay; HYPERLIPIDEMIA
- Publication
Physiological Entomology, 1999, Vol 24, Issue 4, p327
- ISSN
0307-6962
- Publication type
Article
- DOI
10.1046/j.1365-3032.1999.00147.x