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- Title
Coding and noncoding landscape of extracellular RNA released by human glioma stem cells.
- Authors
Zhiyun Wei; Batagov, Arsen O.; Schinelli, Sergio; Jintu Wang; Yang Wang; El Fatimy, Rachid; Rabinovsky, Rosalia; Balaj, Leonora; Chen, Clark C.; Hochberg, Fred; Carter, Bob; Breakefield, Xandra O.; Krichevsky, Anna M.
- Abstract
Tumor-released RNA may mediate intercellular communication and serve as biomarkers. Here we develop a protocol enabling quantitative, minimally biased analysis of extracellular RNAs (exRNAs) associated with microvesicles, exosomes (collectively called EVs), and ribonucleoproteins (RNPs). The exRNA complexes isolated from patient-derived glioma stem-like cultures exhibit distinct compositions, with microvesicles most closely reflecting cellular transcriptome. exRNA is enriched in small ncRNAs, such as miRNAs in exosomes, and precisely processed tRNA and Y RNA fragments in EVs and exRNPs. EV-enclosed mRNAs are mostly fragmented, and UTRs enriched; nevertheless, some full-length mRNAs are present. Overall, there is less than one copy of non-rRNA per EV. Our results suggest that massive EV/exRNA uptake would be required to ensure functional impact of transferred RNA on brain recipient cells and predict the most impactful miRNAs in such conditions. This study also provides a catalog of diverse exRNAs useful for biomarker discovery and validates its feasibility on cerebrospinal fluid.
- Subjects
HUMAN stem cells; TRANSFER RNA; NUCLEOPROTEINS; RNA; CELL communication; CEREBROSPINAL fluid; RNA analysis
- Publication
Nature Communications, 2017, Vol 8, Issue 1, p1
- ISSN
2041-1723
- Publication type
Article
- DOI
10.1038/s41467-017-01196-x