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- Title
Efficient Biosynthesis of Ethyl (R)-3-Hydroxyglutarate through a One-Pot Bienzymatic Cascade of Halohydrin Dehalogenase and Nitrilase.
- Authors
Yao, Peiyuan; Wang, Lei; Yuan, Jing; Cheng, Lihua; Jia, Rongrong; Xie, Meixian; Feng, Jinhui; Wang, Min; Wu, Qiaqing; Zhu, Dunming
- Abstract
An effective one‐pot bienzymatic synthesis of ethyl (R)‐3‐hydroxyglutarate (EHG) from ethyl (S)‐4‐chloro‐3‐hydroxybutyrate (ECHB) was achieved by using recombinant Escherichia coli cells expressing separately or co‐expressing a mutant halohydrin dehalogenase gene from Agrobacterium radiobacter AD1 and a nitrilase gene from Arabidopsis thaliana. The activity of nitrilase was inhibited by high concentration of ECHB and NaCN. Consequently, the one‐pot one‐step process was implemented by fed‐batch of ECHB and NaCN with high accumulative product concentration (up to 0.9 mol L−1). The biotransformation of ECHB to EHG was successfully achieved at 1.2 mol L−1 substrate concentration by a one‐pot two‐step process. As such, this one‐pot bienzymatic transformation should be useful in synthesizing these important optical pure β‐hydroxycarboxylic acids. Team‐up bonus: A novel one‐pot bienzymatic cascade combining halohydrin dehalogenase (HHDH) and nitrilase for the synthesis of ethyl (R)‐3‐hydroxyglutarate from ethyl (S)‐4‐chloro‐3‐hydroxybutyrate is performed at high substrate concentration, and the product was obtained in high yield without separation and purification of the intermediate.
- Subjects
BIOSYNTHESIS; BIOTRANSFORMATION (Metabolism); HALOHYDRINS; NITRILASES; DEHALOGENASES
- Publication
ChemCatChem, 2015, Vol 7, Issue 9, p1389
- ISSN
1867-3880
- Publication type
Interview
- DOI
10.1002/cctc.201500276