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- Title
Increased bone loss and amount of osteoclasts in kinin B1 receptor knockout mice.
- Authors
Gonçalves‐Zillo, Thais Oliveira; Pugliese, Lívia Souza; Sales, Vicência Micheline Toledo; da Silva Mori, Marcelo Alves; Squaiella‐Baptistão, Carla Cristina; Longo‐Maugéri, Ieda Maria; Lopes, José Daniel; Oliveira, Suzana Macedo; Monteiro, Ana Carolina; Pesquero, João Bosco
- Abstract
Aim The pathophysiology of periodontal diseases involves aspects of immunity and bone remodelling. Considering the role of the kinin B1 receptor (Bdkrb1) in inflammation and healing, the purpose of this study was to evaluate the contribution of Bdkrb1 to the pathogenesis of periodontitis. Material and Methods We used a model of ligature-induced experimental periodontitis (LIEP) in mice lacking Bdkrb1 (Bdkrb1−/−) to test the role of this receptor in bone loss and cytokine secretion by lymph nodes cells. Angiotensin-converting enzyme inhibitor (ACEi) was used as a pharmacological strategy to support the genetic model. Also, autonomous effect of Bdkrb1 deletion was evaluated in osteoclasts precursors from bone marrow. Results Bdkrb1−/− mice exhibit increased bone loss and IL-17 secretion in response to LIEP when compared to wild type. LIEP does not modify TNF- α, IFN- γ and IL-10 levels in Bdkrb1−/− mice after 21 days. Bone marrow cells from Bdkrb1−/− displayed increased differentiation into functional osteoclasts with consistent artificial calcium phosphate degradation. Furthermore, treatment of mice with ACEi prevented bone destruction. Conclusion Bdkrb1 participates in the pathogenesis of LIEP bone loss possibly through mechanisms that involve modulation of the TH17 response, thereby demonstrating its role in the development of periodontitis.
- Subjects
BRAZIL; ACE inhibitors; OSTEOPOROSIS genetics; ANIMAL experimentation; CELL receptors; MICE; PERIODONTITIS; RESEARCH funding
- Publication
Journal of Clinical Periodontology, 2013, Vol 40, Issue 7, p653
- ISSN
0303-6979
- Publication type
Article
- DOI
10.1111/jcpe.12097