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- Title
Cytogenetic analysis of hybrids and hybrid triploids between the river puffer, <italic>Takifugu obscurus</italic>, and the tiger puffer, <italic>Takifugu rubripes</italic>.
- Authors
Yoo, Gwang Yeol; Lee, Tae Ho; Gil, Hyun Woo; Lim, Sang Gu; Park, In‐Seok
- Abstract
Abstract: Cytogenetic analyses of the river puffer, <italic>Takifugu obscurus</italic>, the tiger puffer, <italic>Takifugu rubripes</italic> and hybrids produced between female <italic>T. obscurus</italic> and male <italic>T. rubripes</italic> and their hybrid triploids (produced by cold shock treatment at 4°C) were performed. <italic>T. obscurus</italic> had 2<italic>n</italic> = 44 chromosomes and 1.84 ± 0.019 pg DNA/cell, <italic>T. rubripes</italic> had 2<italic>n</italic> = 44 and 2.64 ± 0.015, the hybrids had 2<italic>n</italic> = 44 and 2.15 ± 0.010 and the hybrid triploids had 3<italic>n</italic> = 66 and 3.22 ± 0.010. The erythrocyte values of the hybrids were more similar to those for <italic>T. obscurus</italic>, whereas the hepatocyte, midgut and proximal tubule kidney cell values of the hybrids fell down between those for the parental species (<italic>p </italic><<italic> </italic>.05). The erythrocyte, proximal tubule, hepatocyte and midgut epithelial cell sizes for the hybrid triploids were 1.5‐fold larger than those for the hybrids (<italic>p </italic><<italic> </italic>.05). The thickness of retina and each layer in the hybrid triploids were 1.1‐fold larger than those of the hybrids (<italic>p </italic><<italic> </italic>.05) and did not differ significantly among <italic>T. obscurus</italic>,<italic> T. rubripes</italic> and the hybrids (<italic>p </italic>><italic> </italic>.05); however, the hybrid triploids had fewer cell nucleus outer layers than the hybrids (<italic>p </italic><<italic> </italic>.05). Gonad development in the hybrids and hybrid triploids was less matured than in <italic>T. obscurus</italic> and <italic>T. rubripes</italic>. The metaphase nucleolus organizer regions (NORs) and the gill cells of <italic>T. obscurus</italic>,<italic> T. rubripes</italic> and the hybrids contained two satellite telocentrics, whereas the hybrid triploids contained three satellite telocentrics.
- Subjects
PUFFERS (Fish); BREEDING; GENETICS; SPECIES hybridization; AQUACULTURE
- Publication
Aquaculture Research, 2018, Vol 49, Issue 2, p637
- ISSN
1355-557X
- Publication type
Article
- DOI
10.1111/are.13493