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- Title
Development of a rapid chemiluminescent ciELISA for simultaneous determination of florfenicol and its metabolite florfenicol amine in animal meat products.
- Authors
Tao, Xiaoqi; Yu, Xuezhi; Zhang, Dongdong; Shi, Weimin; Jiang, Haiyang; Wang, Xia; Wang, Zhanhui; Niu, Lanlan; Wu, Xiaoping; Xia, Xi; Shen, Jianzhong
- Abstract
BACKGROUND A rapid one-step chemiluminescent competitive indirect enzyme-linked immunosorbent assay ( CL-ciELISA) for florfenicol ( FF) and its major metabolite florfenicol amine ( FFA) residues in animal meat products has been developed. RESULTS The 50% binding inhibition ( IC50) values of the method were 0.195 µg kg−1 for FFA and 0.24 µg kg−1 for FF under optimum conditions. The cross-reactive rates for FF and FFA were 100.0% and 81.2%, respectively. FF and FFA were easily extracted from animal meat product with an FF/ FFA extraction buffer, obtaining recoveries of 81.8-92.0% ( FF) and 77.2-100% ( FFA). The whole one-step CL-ciELISA test can be accomplished within 40 min in theory. The detection limits ( LODs) of the assay were 0.98 µg kg−1 for FF and 0.80 µg kg−1 for FFA in animal meat samples. Finally, field animal meat samples were analyzed with the CL-ciELISA method, and the results correlated well with those obtained using traditional ELISA and a previously reported liquid chromatographic-tandem mass spectrometric method. CONCLUSION The combined results confirmed the utility of this faster one-step CL-ciELISA for simultaneous trace analysis of FF and FFA. To date, this is the most rapid developed ELISA and CL-ELISA method for detection of FF and FFA. © 2013 Society of Chemical Industry
- Subjects
ENZYME-linked immunosorbent assay; THIAMPHENICOL; TANDEM mass spectrometry; TRACE analysis; LIQUID chromatography; DETECTION limit
- Publication
Journal of the Science of Food & Agriculture, 2014, Vol 94, Issue 2, p301
- ISSN
0022-5142
- Publication type
Article
- DOI
10.1002/jsfa.6258