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- Title
GENETIC STRUCTURE AT PRP LOCUS IN AN OVINE SCRAPIE NUCLEUS OF TURCANA BREED, HATEG ECOTYPE.
- Authors
Viorica, Coşier; Vlaic, A.; Padeanu, I.; Dărăban, S.; Voia, S.; Cătoi, C.; Constantinescu, R.; Vicovan, G.
- Abstract
In the present paper, we share the results of DNA investigations to determine polymorphism of the PrP locus, carried out in an ovine nucleus of Turcana breed, Hateg ecotype, in which scruple has been diagnosed. The PrP locus is polymorph with known variability at codon 136, 154, 171, which are associated with different sensibility in experimental and natural spongiform encephalopaties. Generally, the possible combinations of the 5 amino acids encoded by the 3 different codons will determine the existence of 15 possible genotypes. Biological samples (blood) were collected from 123 males (Tsurcana breed, Hateg ecotype) from herd in Hunedoara County, where scrapie was diagnosed post-mortem by the veterinary authorities. The samples were processed to reveal polymorphism in codons 136, 154 si 171, in a 3130x1 Genetic Analyzer, Applied Biosystem, as described earlier (Coşier et al., 2008). Due to the genetic determination of susceptibility to scruple and other forms of transmissible spongiform encephalopathy (TSE) in sheep breeding to the less susceptible prion protein (PrP) genotype ARR/ARR was advanced within EU. Table 1 presents the allele frequency calculated at the PrP locus in the analyzed population. The results obtained indicate the high proportion (8.94%) of the allele which confers minimum resistance to scrapie (VRQ) and a relatively low frequency of the allele which confers increased resistance-ARR (34.15%) as compared to other breeds (Suffolk-52.4%; Coburg Fox-50.2%, Rhoen-50.12%), which accounts for the occurrence of transmissible spongiform encephalopathy in this nucleus.
- Subjects
GENETICS; EMBRYOLOGY; SCRAPIE; VIRUS diseases; PRIONS; PROTEINS; GOATS
- Publication
Bulletin of the University of Agricultural Sciences & Veterinary Medicine Cluj-Napoca. Animal Science & Biotechnologies, 2008, Vol 65, Issue 1/2, p470
- ISSN
1843-5262
- Publication type
Article