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- Title
黑曲霉解磷能力的影响因素及培养条件优化.
- Authors
孙 冉; 张 素; 吴臣林; 李 祝; 肖 洋
- Abstract
In this study, we investigated factors affecting the phosphate solubilizing capacity of AsgergiZZus niger. A strain named A. niger Xj-2 was isolated by single-spore isolation, with phosphate solubilizing capacity of 539.90 mg •L-1 in liquid medium. The simulated process of fermentation kinetics showed that phosphate concentration reached equilibrium after 4 d cultivation, indicating that the optimal fermentation time was around 4 d. Phosphate solubilization capacity of strain Xj-2 in different phosphorus-source culture media followed the order: calcium phosphate (539.90 mg •L-1) >zinc phosphate (238.45 mg •L-1) > ferric phosphate (182.64 mg • L-1) >phosphate rock power (71.80 mg •L-1) > aluminum phosphate (24.40 mg • L-1). We further examined the optimal condition of strain Xj-2 for phosphate solubilization by response surface optimization combined with single factor experiments. Results showed that the influence of various factors on the phosphate solubilizing capacity of strain Xj-2 was in the order: carbon source > microbial density > pH. The maximum phosphate solubilizing capacity (616.81 mg • L-1) could be achieved under the following condition with 10.00 g • L 'glucose, 0.79 g•L-1 urea, 35 ℉, the initial pH of medium 6.0, 3.8% microbial density and 160 r. min 1 rotating speed.
- Subjects
ALUMINUM phosphate; PHOSPHATES; CALCIUM phosphate; SOLUBILIZATION; FERMENTATION; UREA; GLUCOSE
- Publication
Yingyong Shengtai Xuebao, 2020, Vol 31, Issue 6, p1963
- ISSN
1001-9332
- Publication type
Article
- DOI
10.13287/j.1001-9332.202006.033