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- Title
施用有机肥对侵蚀黑土玉米苗期 根内生细菌多样性的影响.
- Authors
喻 江; 于镇华; IKENAGA Makoto; SAKAI Masao; 刘晓冰; 王光华
- Abstract
In order to investigate the change of root endophytic bacterial communities under soil erosion condition, and to evaluate the response of root endophytic bacteria to manure fertilizer, we adopted the LNA-PCK clamping and 454 bar-coded pyrosequencing methods to study the corn root endophytic bacterial communities under 30 cm topsoil erosion and manure fertilization conditions. No topsoil removing (0 cm) and only chemical fertilizer treatment were used as control. A total of 37820 valid sequences of 16S rDNA were obtained, mainly distributed in 4 phyla, 35 classes, 214 genera and 782 OTUs. The dominant phyla were Proteobacteria, Firmicutes, Actinobacteria and Bacteroidetes, but their proportions varied in different samples. The diversity of corn root endophytic bacteria decreased in soil erosion condition. In the topsoil removing soil and no erosion soil, the diversity of corn root endophytic bacterial communities increased by manure application, and the effect was more obvious in the topsoil removing soil.
- Publication
Yingyong Shengtai Xuebao, 2016, Vol 27, Issue 8, p2663
- ISSN
1001-9332
- Publication type
Article
- DOI
10.13287/j.1001-9332.201608.012