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- Title
Synthesis of hydroxyethylcellulose‐graft‐poly(N, N‐dimethylacrylamide) copolymer by ATRP and as dynamic coating in capillary electrophoresis.
- Authors
Runmiao Yang; Yuhai Liu; Chunzhi Zheng
- Abstract
Hydroxyethylcellulose‐graft‐poly (N, N‐dimethylacrylamide) was synthesized by successive atom transfer radical polymerization (ATRP) of N,N‐dimethylacrylamide (DMA) monomer using HEC‐Br as initiator, CuBr and 5,5,7,12,12,14‐hexamethyl‐1,4,8,11‐tetraazamacrocyclotetradecane (Me6[14]aneN4) as catalyst and ligand, with molar ratio DMA: HEC‐Br (CBr): CuBr: Me6[14]aneN4= 100 : 1 : 1 : 3. HEC–Br macroinitiator was synthesized by esterification of HEC with 2‐bromoisobutyryl bromide. GPC and 1H NMR studies show that the molecular weight of the resulting PDMA increased linearly with the conversion. Within 6 h, the polymerization can reach almost 60% of conversion. The copolymer is applied for the separation of basic proteins in capillary electrophoresis. The results show that this medium has a powerful capability in resisting basic proteins adsorption because the polymer forms noncovalent coating in silica capillaries. With a broad range of pH 2–7, proteins were separated with sufficient efficiencies above 200,000 plates/m. © 2010 Wiley Periodicals, Inc. J Appl Polym Sci, 2010
- Subjects
ATOMS; POLYMERIZATION; ADDITION polymerization; MONOMERS; LIGANDS (Chemistry); MOLECULAR weights
- Publication
Journal of Applied Polymer Science, 2010, Vol 116, Issue 6, p3468
- ISSN
0021-8995
- Publication type
Article